F1F2-selective NMR: Pulse programs
==================================

All pulse programs are in Bruker format.

Overview
--------

Currently, the following pulse programs are available for download:


+-------------------------------------------------+-----------------------------------+---------------------+
| Experiment                                      | Download                          | Comment             |
+=================================================+===================================+=====================+
| F1F2-selective HN(CA)NH                         | :download:`f1f2hncanh`            |                     |
+-------------------------------------------------+-----------------------------------+---------------------+
| F1F2-selective HN(CA)NH                         | :download:`f1f2hncanh3d`          | pseudo-3D version   |
+-------------------------------------------------+-----------------------------------+---------------------+
| F1F2-selective NOESY-[1H, 15N]-HSQC             | :download:`f1f2nNnoesygpsi`       |                     |
+-------------------------------------------------+-----------------------------------+---------------------+
| F1F2-selective NOESY-[1H, 15N]-HSQC             | :download:`f1f2nNnoesygpsi3d`     | pseudo-3D version   |
+-------------------------------------------------+-----------------------------------+---------------------+
| F1F2-selective NOESY-[1H, 13C]-HSQC             | :download:`f1f2nCnoesygp`         |                     |
+-------------------------------------------------+-----------------------------------+---------------------+
| F1F2-selective NOESY-[1H, 13C]-HSQC             | :download:`f1f2nCnoesygp3d`       | pseudo-3D version   |
+-------------------------------------------------+-----------------------------------+---------------------+
| F1F2-selective [15N]-R1ρ relaxation dispersion  | :download:`f1f2Nr1rho`            |                     |
+-------------------------------------------------+-----------------------------------+---------------------+
| F1F2-selective [15N]-R1ρ relaxation dispersion  | :download:`f1f2Nr1rho3d`          | pseudo-3D version   |
+-------------------------------------------------+-----------------------------------+---------------------+
| F1F2-selective [15N]-R1ρ relaxation dispersion  | :download:`f1f2Nonr1rho4d`        | pseudo-4D version   |
+-------------------------------------------------+-----------------------------------+---------------------+
| F1F2-selective [15N]-ZZ exchange                | :download:`f1f2Nzzhsqcgpsi`       |                     |
+-------------------------------------------------+-----------------------------------+---------------------+


F1F2-selective NMR spectroscopy
-------------------------------

The F1F2-selective pulse sequences are introduced in these publications:

| Walinda, E., Morimoto, D., Shirakawa, M., and Sugase K.,
| F1F2-selective NMR spectroscopy 
| J Biomol NMR 2017, 68(1), 41–52.
|
| https://link.springer.com/article/10.1007%2Fs10858-017-0113-x

| Nishizawa, M., Walinda, E., Morimoto, D., and Sugase, K.
| Pinpoint analysis of a protein in slow exchange using F1F2-selective ZZ-exchange spectroscopy: assignment and kinetic analysis. 
| J Biomol NMR 2020, in press
|
| https://link.springer.com/article/10.1007/s10858-020-00309-x

Please feel free to inquire, if you have questions regarding
the setup of these experiments.


F1F2-selective HN(CA)NH 
-----------------------

This pulse program is a F1F2-selective HN(CA)NH experiment to probe a single
resonance. For the pseudo-3D version, which acquires 2D spectra for multiple
residues, please see the next section.

You can download the pulse program here - :download:`f1f2hncanh`

.. literalinclude:: f1f2hncanh

F1F2-selective HN(CA)NH (pseudo-3D version) 
-------------------------------------------

This pulse program is a F1F2-selective HN(CA)NH experiment to probe multiple
residues as a pseudo-3D acquisition experiment.

You can download the pulse program here - :download:`f1f2hncanh3d`

.. literalinclude:: f1f2hncanh3d




F1F2-selective NOESY-[1H, 15N]-HSQC
-----------------------------------

You can download the pulse program here - :download:`f1f2nNnoesygpsi`

.. literalinclude:: f1f2nNnoesygpsi


F1F2-selective NOESY-[1H, 15N]-HSQC (pseudo-3D version)
-------------------------------------------------------

You can download the pulse program here - :download:`f1f2nNnoesygpsi3d`

.. literalinclude:: f1f2nNnoesygpsi3d



F1F2-selective NOESY-[1H, 13C]-HSQC
-----------------------------------

You can download the pulse program here - :download:`f1f2nCnoesygp`

.. literalinclude:: f1f2nCnoesygp


F1F2-selective NOESY-[1H, 13C]-HSQC (pseudo-3D version)
-------------------------------------------------------

You can download the pulse program here - :download:`f1f2nCnoesygp`

.. literalinclude:: f1f2nCnoesygp3d



F1F2-selective [15N]-R1ρ relaxation dispersion
----------------------------------------------

You can download the pulse program here - :download:`f1f2Nr1rho`

.. literalinclude:: f1f2Nr1rho


F1F2-selective [15N]-R1ρ relaxation dispersion (pseudo-3D version)
------------------------------------------------------------------

You can download the pulse program here - :download:`f1f2Nr1rho3d`

.. literalinclude:: f1f2Nr1rho3d


F1F2-selective [15N]-R1ρ relaxation dispersion (pseudo-4D version)
------------------------------------------------------------------

You can download the pulse program here - :download:`f1f2Nonr1rho4d`

.. literalinclude:: f1f2Nonr1rho4d


F1F2-selective [15N]-ZZ exchange
--------------------------------

You can download the pulse program here - :download:`f1f2Nzzhsqcgpsi`

.. literalinclude:: f1f2Nzzhsqcgpsi